Bacterial strains with cellulolytic and xylanolytic activity originated from different isolation sources

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Structural polysaccharide degrading bacteria can potentially be used in different biotechnological processes for the initiation of enzymatic decay in plant biomass. These cellulolytic and xylanolytic bacteria represent valuable sources of hydrolytic enzymes. Our aim was to isolate and characterize bacterial strains, which contribute to the increase of water soluble carbohydrate content in different raw materials of plant biomass. Due to their beneficial effect, these bacteria can improve the hydrolysis rate of lignocellulose biomass. During our work, a number of 208 bacterial isolates with cellulose and hemicellulose-degrading capacity have been obtained. The selected strains have been assayed for cellulolytic and xylanolytic activities. 47 isolates with structural polysaccharide degrading capacity have been identified based on their16S ribosomal DNA gene sequence analysis. The identified bacterial strains belong to different genera such as: Bacillus, Stenotrophomonas, Acinetobacter, Paenibacillus, Comamonas, Corynebacterium, Flavobacterium, Janthinobacterium, Kurthia, Leucobacter, Pseudomonas, and Sphingobacterium.